Ditulis pada tanggal 11 July 2017, oleh staff psik 1, pada kategori jurnal

ANTIBACTERIAL EFFECT EXPLORATIAN OF Holothuriascabra AGAINTS

Aeromonas hydrophila BACTERIA
Maftuch1
Lecturer of Fisheries and Marine Science University of Brawijaya
Abstract
This research was conducted to study the effect of Holothuriascabra antibacterial againstA. hydrophila bacteria. The
Holothuriascabra antibacterial was extracted with Metanol 97% and followed by rotary evaporator and water bath until
become gel extract. Based on phithochamical test, Holothuriascabracontains saponin and triterpenoid. The method used in
this research was the separation of Holothuriascabra fractions using column chromatography obtained seven fractions.
Three fractions resulted best inhibition test were EtilAsetat :Cloroform (5,53 mm); Etilasetat : Metanol (4,8 mm); EtilAsetat
: Metanol (4,54 mm). The following tested to detect each wave lenght by UV VIS were resulted 200-250 nm.
Keywords: Antibacterial, Holothuriascabra, Ahydrophila.

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