Ditulis pada tanggal 11 July 2017, oleh staff psik 1, pada kategori jurnal

Antibacterial effect of marine algae (Gracilaria verrucosa) againstVibrio harveyi
andVibrio algynolyticus
Maftuch1*
1Laboratory of Fish Diseases and Parasite, Faculty of Fisheries and Marine Science, University of
Brawijaya, Indonesia.
Corresponding author: maftuch2@gmail.com
Abstract
The antibacterial effect of extract subtance has been established in many systems including
fish. An investigationwas carried out to study the effect of antibacterial marine algae (Gracilaria
verrucosa) extract against pathogenic bacteria. The marine algae (Gracilaria verrucosa) was extracted
with 96% etanol, followed by rotary evaporator and water bath until became crude extract
gell.Based on phitochemical tested,Gracilaria verrucosa crude extract contains alkaloid, flavonoid,
tannin, steroid, saponin and fenol. This extract was tested against the pathogen Vibrio harveyi
isolated from the infected black tiger shrimp and Vibrio algynolyticus isolated from grouper.
Antibacterial parameterwas in vitro inhibition zone of bacterial growth. The dosage of extract
was 1 ppt (1 gr : 1 ml DMSO 10%). All data were collected 48 hours after incubation treatments.
The results showed that,compared with those in control, two treatments resulted in significant
inhibition. Inhibition zone of Vibrio harveyi was 7.75 mm and Vibrio algynolyticus was 4.0 mm.
These results indicated that the extract of marine algae has strong inhibition at Vibrio harveyi from
black tiger shrimp (Penaeus monodon Fabricus).
Key words: antibacterial, Gracilaria verrucosa, Vibrio harveyi,Vibrio algynolyticus, black tiger shrimp,
Grouper.

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