Carrageenan from Euchema sp and concetration difference as

encapsulation material toward Lactobacillus acidophilus viability at simulation GI Tract pH condition

Dwi Setijawati

Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java of Indonesia

ISSN 2090-4304

Receive : April 6,2014 and Accepted : June 17, 2014

ABSTRACT

Euchema cottonii species produce kappa and Euchema spinosum species produce iota carrageenan. Kappa gave strong, rigid, syneresi, but Iota soft, elastic and no syneresis gel properties. Mixture both them material improved gel properties. So usage of them as encapsulation material influenced Lactobacillus acidophilus viability. Aimed of this research was study influence and find the best treatment from carrageenan as encapsulation material with species and concentration difference toward Lactobacillus acidophilus viability in simulation GI Tract Ph condition. The method was experimental laboratory design. Material was :1) carrageenan from species Euchema cottonii and Euchema spinosum harvested by lombok island in 42 days; 2) Lactobacillus acidophilus microcapsule, RAL factorial design, data analysed by minitab 16 software. Treatmant was A1=kappa; A2=iota; A3=KAPPA: IOTA (75:25); A4= kappa: iota (50:50); B1=4,5%; B2=6%. Enumeration viability used MRS agar pour plate method. The result was species and concentration gave different influence toward Lactobacillus acidiphilus viability through simulation GI Tract ph condition. The best result was mix kappa-iota carrageenan (75:25), 6% concentration with the highest viability average 6, 1097 cfu/ml (log). The standard of benefit value for health was 107 cfu/ml viability, but must to increased it’s concentration to fulfill the standard of benefit value for health.

KEYWORDS : Euchema cottonii, Euchema spinosum, microencapsulation, emulsification

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