Verification on Morphological Identification of Grapsid Crabs Genus Metopograpsus H.

Milne-Edwards, 1853 from Chon Buri Province using Partial Sequences of Mitochondrial

16S rRNA, 12S rRNA and Cytochrome c Oxidase Subunit I (COI) genes



We analyzed the partial sequences of mitochondrial 16S rRNA, 12S rRNA and Cytochrome c oxidase subunit I (COI) genes to discriminate three morphological species of grapsid crabs, Metopograpsus oceanicus (Hombron and Jacquinot, 1846), M. frontalis (Miers, 1880) and M. latifrons (White, 1847). All genetic markers were informative for species identification. Interspecific nucleotide divergence among these species ranged from 5.8% to 7.9% (16S rRNA and 12S rRNA genes) and from 8.7% to 12.3% (COI gene). Intraspecific divergence levels ranged from 0.0% to 1.9%. Phylogenetic analyses based on all approaches revealed clear differentiation among the three Metopograpsus species (bootstrap values = 50 to 100). Mitochondrial 16S rRNA and 12S rRNA gene sequences suggested M. frontalis and M. latifrons were closely related by Maximum Likelihood, ML; Neighbor Joining, NJ; Maximum Parsimony, MP analyses. However, COI gene sequence suggested that M. frontalis and M. oceanicus were more closely related.

Keywords: 16S rRNA gene, 12S rRNA gene, Cytochrome c oxidase subunit I, species identification, Metopograpsus

Jurnal Ade Yamindago