Collagen Extraction from Tuna’s Skin (Thunnus sp) Waste with Various concentrations of NaCl

Happy Nursyam

Lecturer in the Fisheries and Marine Sciences Faculty, Universitas Brawijaya

ABSTRACT

The purpose of this research is to study the extraction methods and collagen physical properties which is obtained through isolation with various concentrations of NaCl, those are; 0,9M; 1,1M; 1.3m; 1.5m; and 1,7M. This study was done descriptively with using parameters that include:  yield, water content, melting point, electrophoresis and SEM. From the research results are as follows: (1) To extract the best collagen in this study is using of NaCl 1,7M with a yield of 45.06 + – 0.644%, water content 82.561 + – 0.217% and melting point 39.667 + – 0.577 C and (2) the collagen fibers that are formed in the 1,7M NaCl concentration are thick, tightly and not many cavities while the 0,9M NaCl concentration are thin and many cavities. Based on the protein bands analysis by SDS-PAGE, it is essential to study more about collagen extracted using NaCl 0,9M because having the amino acid tyrosine.

Keywords: collagen, extraction, tuna skin waste, electrophoresis and SEM.

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